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KMID : 0384119930130040559
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Korean Journal of Clinical Pathology
1993 Volume.13 No. 4 p.559 ~ p.568
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Detection of Rearrangements of the PML and the Retinoic Acid Receptor Alpha Genes Using Reverse Transcription Polymerase Chain Reaction in Patients with Acute Promyelocytic Leukemia
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Abstract
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The characteristic t(15;17) of acute promyelocytic leukemia(AML, M3:APL) fuses the retinoic acid receptor alpha(RARA) gene on chromosome 17 to the PML gene on chromosome 15. These rearrangements result in PML-RARA fusion message which may be
important
in leukemogenesis of APL and in the treatment with all-trans retinoic acid. We tried to detect rearrangements of PML and RARA in Korean patients with APL using reverse transcription polymerase chain reactions(RT-PCR) which were designed to
amplify
either PML-RARA(15q+ derived) or RARA-PML(17q- derived) chimeric transcripts. Both PML- RARA and RARA-PML rearrangements were detected in all of five cases with APL, and the breakpoint analysis of PML suggested that three of five cases had 3'
breakpoints(probably intron 6) and one case had 5' breakpoint(probably intron 3). This study suggests that RT0PCR can be used in the diagnosis and therapeutic monitoring of APL, whereas more precise analysis of breakpoints within the PML gene is
necessary for the optimization of molecular diagnosis of APL.
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KEYWORD
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